Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts
Skeletal myoblasts undergo a well-characterized sequence of
morphological and transcriptional changes during differentiation. In
this experiment, primary human skeletal muscle myoblasts (HSMM) were
expanded under high mitogen conditions (GM) and then differentiated by
switching to low-mitogen media (DM). RNA-Seq libraries were sequenced
from each of several hundred cells taken over a time-course of serum-
induced differentiation. Between 49 and 77 cells were captured at each
of four time points (0, 24, 48, 72 hours) following serum switch using
the Fluidigm C1 microfluidic system. RNA from each cell was isolated and
used to construct mRNA-Seq libraries, which were then sequenced to a
depth of ~4 million reads per library, resulting in a complete gene
expression profile for each cell.
